
Additional resources for using your microscope expertly:
https://www.zeiss.com/microscopy/int/service-support/training-and-education.html
https://www.nikoninstruments.com/Learn-Explore
https://www.olympus-ims.com/en/resources/
https://www.microscopyu.com/
Get you quickly to producing simple algorithms, without getting bogged down by more information than you need
Give you a broad, working mental model of image analysis algorithms, to facilitate further learning of more detailed information
Experiment, come up with your own solutions, learn by trial-and-error.
Basic thresholding
Recognizing when noise reduction would be helpful
Segmentation cleanup
Simple measurements
Planning desired selections
Comparing types of noise reduction
Considerations when choosing how to clean a segmentation
Noticing variation in your image
Dealing with variation in your image
•During pre-processing
•During segmentation
Measurement interpretation: trusting numbers in isolation
Recognizing features in an image that can be targeted with filters
Compensating for filter side-effects
Check your measurements
Proper image illumination
Being aware of filter side-effects
Combining multiple segmentations
Using segmentations as masks for subsequent processing
Identify features that might confound your segmentation
Ways to handle these features, depending on how they interfere
•Grayscale pre-processing vs. segmentation mathematics
2-stage segmentation: What defines a feature vs. what differentiates a feature from others
Identify errors in your segmentation and whether those errors need to be addressed
Masks and Regions of Interest
Centers vs edges
Be OK with having errors in your first pass at segmentation.
Understand the strengths of different filters and segmentation methods, so you can apply them where appropriate
•Also, being willing to experiment
How to double-check your work
Memory management
How morphological operations will affect different parts of your segmentation
Continued two-stage segmentation / define vs. distinguish
•Returning to your original segmentation to reduce side-effects
Segmentation mathematics to combine segmentations and achieve optimal results
Measuring one segmentation relative to another
Understand needed segmentation accuracy
Consider potential variation between your images
Deciding the best way to measure a feature
In this course you will learn how to create image analysis algorithms that actually work. You will learn the basics of image analysis, how to create your own analysis workflows, and how to become an expert at solving your own problems. We will teach you all the tools required to go from beginner to expert in image analysis. Come join us!
Note: We have lots more content planned! The finished course will have five sections, each covering a stage of the image analysis process. Be sure to stay tuned!